Proteintech, 18322-1-AP, MAD1 Polyclonal antibody

Size: 20ul / 150ul The MAD1 (18322-1-AP) by Proteintech is a Polyclonal antibody targeting MAD1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 18322-1-AP targets MAD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, human brain tissue, Raji cells, HeLa cells, HepG2 cells Positive IP detected in: HeLa cells Positive IHC detected in: human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells, SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MAD1L1 is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. It recruits MAD2L1 to unattached kinetochores. Also it is required for anchoring MAD2L1 to the nuclear periphery. In addition, it binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding [PMID:10049595, 12837246]. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13171 Product name: Recombinant human MAD1L1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 370-718 aa of BC009964 Sequence: QEELRQVSGQLLEERKKRETHEALARRLQKRVLLLTKERDGMRAILGSYDSELTPAEYSPQLTRRMREAEDMVQKVHSHSAEMEAQLSQALEELGGQKQRADMLEMELKMLKSQSSSAEQSFLFSREEADTLRLKVEELEGERSRLEEEKRMLEAQLERRALQGDYDQSRTKVLHMSLNPTSVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKEVAELKKQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQIDITTENQYRLTSLYAEHPGDCLIFKATSPSGSKMQLLETEFSHTVGELIEVHLRRQDSIPAFLSSLTLELFSRQTVA Predict reactive species Full Name: MAD1 mitotic arrest deficient-like 1 (yeast) Calculated Molecular Weight: 83 kDa Observed Molecular Weight: 83 kDa GenBank Accession Number: BC009964 Gene Symbol: MAD1 Gene ID (NCBI): 8379 RRID: AB_2139251 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y6D9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.