Proteintech, 18708-1-AP, Cytokeratin 18 Polyclonal antibody

Size: 20ul / 150ul The Cytokeratin 18 (18708-1-AP) by Proteintech is a Polyclonal antibody targeting Cytokeratin 18 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 18708-1-AP targets Cytokeratin 18 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, COLO 320 cells, HepG2 cells Positive IP detected in: A431 cells Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human endometrial cancer tissue, human kidney tissue, human liver tissue, human nephroblastoma tissue, human ovary tumor tissue, human renal cell carcinoma tissue, human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:300-1:1200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. This antibody is specifically agianst KRT18. Specification Tested Reactivity: human Cited Reactivity: mouse, pig, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: keratin 18 Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 48 kDa GenBank Accession Number: BC000180 Gene Symbol: Cytokeratin 18 Gene ID (NCBI): 3875 RRID: AB_2133162 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P05783 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.