Proteintech, 18721-1-AP, GABARAPL1-Specific Polyclonal antibody

Size: 20ul / 150ul The GABARAPL1-Specific (18721-1-AP) by Proteintech is a Polyclonal antibody targeting GABARAPL1-Specific in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 18721-1-AP targets GABARAPL1-Specific in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, human heart tissue, human spleen tissue, mouse kidney tissue, mouse liver tissue, rat brain tissue Positive IHC detected in: human kidney tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle. And at very low levels in the thymus and small intestine. The antibody is specific to GABARAPL1 and doesn't recognize the recombinant GABARAP and GABARAPL2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: GABA(A) receptor-associated protein like 1 Calculated Molecular Weight: 14 kDa Observed Molecular Weight: 17 kDa GenBank Accession Number: NM_031412 Gene Symbol: GABARAPL1 Gene ID (NCBI): 23710 RRID: AB_10597556 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H0R8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.