Proteintech, 18736-1-AP, HOXD13 Polyclonal antibody

Size: 20ul / 150ul The HOXD13 (18736-1-AP) by Proteintech is a Polyclonal antibody targeting HOXD13 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 18736-1-AP targets HOXD13 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, rat brain tissue, Jurkat cells, mouse lung tissue, mouse brain tissue Positive IP detected in: A549 cells Positive IHC detected in: human brain tissue, human hepatocirrhosis tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information HOXD13, also named as HOX4I, belongs to the Abd-B homeobox family. HOXD13 is a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Mutation of HOXD13 will cause synpolydactyly (SPD), brachydactyly type D (BDD), syndactyly type 5brachydactyly-syndactyly syndrome (BDSD) or brachydactyly type E (BDE).Present polyclonal anti-HOX13D antibody(18736-1-AP) is produced by immunizing animals with part of N-terminus chain of HOX13D and detect a 36-kDa band in cell and tissues. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: homeobox D13 Calculated Molecular Weight: 343 aa, 36 kDa Observed Molecular Weight: 36 kDa GenBank Accession Number: BC148863 Gene Symbol: HOXD13 Gene ID (NCBI): 3239 RRID: AB_2119926 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P35453 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.