Proteintech, 18837-1-AP, HMCN1 Polyclonal antibody

Size: 20ul / 150ul The HMCN1 (18837-1-AP) by Proteintech is a Polyclonal antibody targeting HMCN1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 18837-1-AP targets HMCN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse skin tissue Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: hTERT-RPE1 cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Hemicentin-1 (HMCN1), also known as fibulin-6 (FIBL-6), is an extracellular protein of the immunoglobulin superfamily. HMCN1 consists of a von Willebrand A domain, tandem immunoglobulin modules, tandem EGF modules, and a fibulin carboxy-terminal module (PMID: 24912920). Mutations of HMCN1 gene may be associated with age-related macular degeneration. Three alternative transcripts encoding different HMCN1 isoforms have been described. This antibody raised against a synthesized peptide corresponding to 960-976 amino acids of human HMCN1 can recognize all three isoforms of HMCN1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: hemicentin 1 Calculated Molecular Weight: 613 kDa, 600 kDa, 60 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: NM_031935 Gene Symbol: HMCN1 Gene ID (NCBI): 83872 RRID: AB_2878563 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96RW7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.