Proteintech, 18986-1-AP, EGFR-Specific Polyclonal antibody

Size: 20ul / 150ul The EGFR-Specific (18986-1-AP) by Proteintech is a Polyclonal antibody targeting EGFR-Specific in WB, IP, ELISA applications with reactivity to human samples 18986-1-AP targets EGFR-Specific in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, MCF-7 cells, A431 cells, HeLa/RPE1 cells, NCI-H1299 cells, HeLa cells, A549 cells, L02 cells, NCL-H1299 cells Positive IP detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, pig, sheep, goat, sus scrofa (pig) Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Calculated Molecular Weight: 134 kDa Observed Molecular Weight: 145-165 kDa GenBank Accession Number: NM_005228 Gene Symbol: EGFR Gene ID (NCBI): 1956 RRID: AB_10596476 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P00533 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.