Proteintech, 18987-1-AP, DOCK9 Polyclonal antibody

Size: 20ul / 150ul The DOCK9 (18987-1-AP) by Proteintech is a Polyclonal antibody targeting DOCK9 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 18987-1-AP targets DOCK9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, human brain tissue, mouse brain tissue, human heart tissue, human placenta tissue, HeLa cells, MCF-7 cells Positive IP detected in: mouse brain tissue Positive IHC detected in: human kidney tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information DOCK9 is a guanine nucleotide exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Its overexpression induces filopodia formation. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: dedicator of cytokinesis 9 Calculated Molecular Weight: 239 kDa Observed Molecular Weight: 200-236 kDa GenBank Accession Number: NM_015296 Gene Symbol: DOCK9 Gene ID (NCBI): 23348 RRID: AB_10596777 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BZ29 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.