Proteintech, 19510-1-AP, PTPN20 Polyclonal antibody

Size: 20ul / 150ul The PTPN20 (19510-1-AP) by Proteintech is a Polyclonal antibody targeting PTPN20 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 19510-1-AP targets PTPN20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: mouse tongue tissue, pig tongue tissue, rat tongue tissue Positive IHC detected in: mouse tongue tissue, mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PTPN20B, also named as PTPN20A, belongs to the protein-tyrosine phosphatase family and Non-receptor class subfamily. PTPN20B is a tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. PTPN20B has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates. PTPN20B catalyze the reaction: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. The antibody can recognize the isoform 1,2,7,8,10,11,13,15. Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: protein tyrosine phosphatase, non-receptor type 20B Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: NM_015605 Gene Symbol: PTPN20B Gene ID (NCBI): 26095 RRID: AB_3669310 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q4JDL3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.