Proteintech, 19555-1-AP, RUNX1 (N-terminal) Polyclonal antibody

Size: 20ul / 150ul The RUNX1 (N-terminal) (19555-1-AP) by Proteintech is a Polyclonal antibody targeting RUNX1 (N-terminal) in WB, IP, ELISA applications with reactivity to human, mouse samples 19555-1-AP targets RUNX1 (N-terminal) in WB, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse liver tissue Positive IP detected in: mouse liver tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists in some isoforms with a range of MV 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa, but the modified protein is about 49-55 kDa. This antibody (19555-1-AP) can't recognize 37 kDa isoform 11. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: runt-related transcription factor 1 Calculated Molecular Weight: 51 kDa Observed Molecular Weight: 48-55 kDa GenBank Accession Number: NM_001754 Gene Symbol: RUNX1 Gene ID (NCBI): 861 RRID: AB_10949190 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q01196 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.