Proteintech, 19743-1-AP, SLK Polyclonal antibody

Size: 20ul / 150ul The SLK (19743-1-AP) by Proteintech is a Polyclonal antibody targeting SLK in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 19743-1-AP targets SLK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, human brain tissue, MCF-7 cells, mouse liver tissue, HeLa cells, THP-1 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information SLK, also named as KIAA0204 and STK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. SLK plays an important role in cell proliferation and cytoskeletal remodeling. In fibroblasts, SLK has been shown to respond to FAK/Src signaling and regulate focal adhesion turnover through Paxillin phosphorylation. Full-length SLK (~220 kDa) has also been shown to be essential for embryonic development. In myoblasts, the overexpression of a dominant negative SLK is sufficient to block myoblast fusion. (PMID: 28153048, PMID: 25882817, PMID: 23815977) Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: STE20-like kinase (yeast) Calculated Molecular Weight: 143 kDa Observed Molecular Weight: 143-160 kDa, 210-220 kDa GenBank Accession Number: NM_014720 Gene Symbol: SLK Gene ID (NCBI): 9748 RRID: AB_10644278 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H2G2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.