Proteintech, 19762-1-AP, GPR50 Polyclonal antibody

Size: 20ul / 150ul The GPR50 (19762-1-AP) by Proteintech is a Polyclonal antibody targeting GPR50 in IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse samples 19762-1-AP targets GPR50 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Positive IF/ICC detected in: SH-SY5Y cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:100-1:500 Background Information GPR50, also known as Melatonin-related receptor or H9, is a member of the G-protein coupled receptor 1 family. It is closely related to the G protein-coupled melatonin receptor family and has been demonstrated as the mammalian ortholog of Mel1c receptor (PMID: 8647286; 18400093). GPR50 does not bind melatonin. It is able to heterodimerize with both the MT1 (Mel1a) and MT2 (Mel1b) melatonin receptor subtypes. While GPR50 has no effect on MT2 function, GPR50 prevented MT1 from both binding melatonin and coupling to G proteins. GPR50 is involved in neuroendocrine function and plays a role in the regulation of energy metabolism (PMID: 8647286; 17957037). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: G protein-coupled receptor 50 Calculated Molecular Weight: 67 kDa GenBank Accession Number: NM_004224 Gene Symbol: GPR50 Gene ID (NCBI): 9248 RRID: AB_2878603 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13585 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.