Proteintech, 19807-1-AP, THUMPD3 Polyclonal antibody

Size: 20ul / 150ul The THUMPD3 (19807-1-AP) by Proteintech is a Polyclonal antibody targeting THUMPD3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 19807-1-AP targets THUMPD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, mouse kidney tissue, L02 cells Positive IHC detected in: human hepatocirrhosis tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Hela cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information THUMPD3, or THUMP domain containing 3, is a protein-coding gene that plays a significant role in tRNA methylation, a post-transcriptional modification that affects tRNA biology and is closely associated with human diseases. THUMPD3 is responsible for catalyzing the formation of N2-methylguanosine (m2G) at position 6 in a broad range of tRNA substrates containing the characteristic 3'-CCA terminus of mature tRNAs. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13833 Product name: Recombinant human THUMPD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 161-507 aa of BC001622 Sequence: GQEVKIDQRNVKKEFTSHALDSHILDYYENPAIKEDVSTLIGDDLASCKDETDESSKEETEPQVLKFRVTCNRAGEKHCFTSNEAARDFGGAVQDYFKWKADMTNFDVEVLLNIHDNEVIVGIALTEESLHRRNITHFGPTTLRSTLAYGMLRLCDPLPYDIIVDPMCGTGAIPIEGATEWSDCFHIAGDNNPLAVNRAANNIASLLTKSQIKEGKPSWGLPIDAVQWDICNLPLRTGSVDIIVTDLPFGKRMGSKKRNWNLYPACLREMSRVCTPTTGRAVLLTQDTKCFTKALSGMRHVWRKVDTVWVNVGGLRAAVYVLIRTPQAFVHPSEQDGERGTLWQCKE Predict reactive species Full Name: THUMP domain containing 3 Calculated Molecular Weight: 507 aa, 57 kDa Observed Molecular Weight: 57 kDa GenBank Accession Number: BC001622 Gene Symbol: THUMPD3 Gene ID (NCBI): 25917 RRID: AB_10644140 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BV44 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.