Proteintech, 19892-1-AP, ASUN Polyclonal antibody

Size: 20ul / 150ul The ASUN (19892-1-AP) by Proteintech is a Polyclonal antibody targeting ASUN in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 19892-1-AP targets ASUN in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, Jurkat cells Positive IP detected in: MCF-7 cells Positive IHC detected in: human cervical cancer tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization duringDrosophilaspermatogenesis (PMID: 19357193). Mammalian andDrosophilaASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13727 Product name: Recombinant human C12orf11 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 357-706 aa of BC003081 Sequence: GRSVLLEQPRKSGSKVISHMLSSHGGEIFLHVLSSSRSILEDPPSISEGCGGRVTDYRITDFGEFMRENRLTPFLDPRYKIDGSLEVPLERAKDQLEKHTRYWPMIISQTTIFNMQAVVPLASVIVKESLTEEDVLNCQKTIYNLVDMERKNDPLPISTVGTRGKGPKRDEQYRIMWNELETLVRAHINNSEKHQRVLECLMACRSKPPEEEERKKRGRKREDKEDKSEKAVKDYEQEKSWQDSERLKGILERGKEELAEAEIIKDSPDSPEPPNKKPLVEMDETPQVEKSKGPVSLLSLWSNRINTANSRKHQEFAGRLNSVNNRAELYQHLKEENGMETTKNGKASRQ Predict reactive species Full Name: chromosome 12 open reading frame 11 Calculated Molecular Weight: 706 aa, 80 kDa Observed Molecular Weight: 70-80 kDa GenBank Accession Number: BC003081 Gene Symbol: ASUN Gene ID (NCBI): 55726 RRID: AB_10638473 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NVM9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.