Proteintech, 19909-1-AP, SLC10A3 Polyclonal antibody

Size: 20ul / 150ul The SLC10A3 (19909-1-AP) by Proteintech is a Polyclonal antibody targeting SLC10A3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 19909-1-AP targets SLC10A3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: 37°C incubated A549 cells Positive IHC detected in: human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The solute carrier (SLC) family 10 (SLC10) encodes protein for transporters of various agents, bile acids and steroidal hormones, and SLC01A3 belongs to the SLC10 family. SLC10A3 consists of 477 amino acids, with a calculated mass of approximately 50 kDa. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13915 Product name: Recombinant human SLC10A3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-185 aa of BC004966 Sequence: MVLMQDKGSSQQWPGLGGEGGGTGPLSMLRAALLLISLPWGAQGTASTSLSTAGGHTVPPTGGRYLSIGDGSVMEFEFPEDSEGIIVISSQYPGQANRTAPGPMLRVTSLDTEVLTIKNLVDAHEAPPTLIEERRDFCIKVSPAEDTPATLSADLAHFSENPILYLLLPLIFVNKCSFGCKVELE Predict reactive species Full Name: solute carrier family 10 (sodium/bile acid cotransporter family), member 3 Calculated Molecular Weight: 477 aa, 50 kDa Observed Molecular Weight: 50 kDa, 48 kDa GenBank Accession Number: BC004966 Gene Symbol: SLC10A3 Gene ID (NCBI): 8273 RRID: AB_10642950 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P09131 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.