Proteintech, 19962-1-AP, ITPR1-specific Polyclonal antibody

Size: 20ul / 150ul The ITPR1-specific (19962-1-AP) by Proteintech is a Polyclonal antibody targeting ITPR1-specific in WB, IHC, IF-Fro, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 19962-1-AP targets ITPR1-specific in WB, IHC, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, mouse brain tissue, mouse liver tissue, L02 cells, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human brain tissue, human testis tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-Fro detected in: mouse brain tissue Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-FRO: IF-FRO : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, chicken, bovine, sheep, duck Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: inositol 1,4,5-triphosphate receptor, type 1 Calculated Molecular Weight: 314 kDa Observed Molecular Weight: 290-300 kDa GenBank Accession Number: NM_001099952 Gene Symbol: ITPR1 Gene ID (NCBI): 3708 RRID: AB_10638786 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14643 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.