Proteintech, 19989-1-AP, LDHC-Specific Polyclonal antibody

Size: 20ul / 150ul The LDHC-Specific (19989-1-AP) by Proteintech is a Polyclonal antibody targeting LDHC-Specific in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 19989-1-AP targets LDHC-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human testis tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue, A549 cells, rat testis tissue Positive IP detected in: mouse testis tissue Positive IHC detected in: mouse testis tissue, human testis tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Hela cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: lactate dehydrogenase C Calculated Molecular Weight: 36 kDa Observed Molecular Weight: 30-36 kDa GenBank Accession Number: NM_002301 Gene Symbol: LDHC Gene ID (NCBI): 3948 RRID: AB_10637847 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P07864 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.