Proteintech, 20002-1-AP, TP53BP1-Specific Polyclonal antibody

Size: 20ul / 150ul The TP53BP1-Specific (20002-1-AP) by Proteintech is a Polyclonal antibody targeting TP53BP1-Specific in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 20002-1-AP targets TP53BP1-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, C6 cells, NIH/3T3 cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage. This antibody is specific to TP53BP1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tumor protein p53 binding protein 1 Calculated Molecular Weight: 214 kDa Observed Molecular Weight: 450 kDa GenBank Accession Number: NM_005657 Gene Symbol: TP53BP1 Gene ID (NCBI): 7158 RRID: AB_3085592 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q12888 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.