Proteintech, 20018-1-AP, NCOR1 Polyclonal antibody

Size: 20ul / 150ul The NCOR1 (20018-1-AP) by Proteintech is a Polyclonal antibody targeting NCOR1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 20018-1-AP targets NCOR1 in WB, IHC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, K-562 cells Positive IHC detected in: human cervical cancer tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information NCOR1, also named as KIAA1047 and TRAC1, belongs to the N-CoR nuclear receptor corepressors family. NCO1 mediates transcriptional repression by certain nuclear receptors. It is part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. The antibody recognizes the 103 kDa isoform of NCOR1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: nuclear receptor co-repressor 1 Calculated Molecular Weight: 270 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: NM_006311 Gene Symbol: NCOR1 Gene ID (NCBI): 9611 RRID: AB_10665360 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75376 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.