Proteintech, 20028-1-AP, MED12 Polyclonal antibody

Size: 20ul / 150ul The MED12 (20028-1-AP) by Proteintech is a Polyclonal antibody targeting MED12 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 20028-1-AP targets MED12 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Mediator of RNA polymerase II transcription subunit 12 (MED12), also named Activator-recruited cofactor 240 kDa component, Thyroid hormone receptor-associated protein complex 230 kDa component. MED12 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Molecular weight of MED12 is about 240kd.This antibody is specific to MED12. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mediator complex subunit 12 Calculated Molecular Weight: 243 kDa Observed Molecular Weight: 243 kDa GenBank Accession Number: NM_005120 Gene Symbol: MED12 Gene ID (NCBI): 9968 RRID: AB_10644141 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q93074 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.