Proteintech, 20177-1-AP, TBC1D9B Polyclonal antibody

Size: 20ul / 150ul The TBC1D9B (20177-1-AP) by Proteintech is a Polyclonal antibody targeting TBC1D9B in WB, IP, ELISA applications with reactivity to human samples 20177-1-AP targets TBC1D9B in WB, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells Positive IP detected in: Jurkat cells, HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information TBC1 domain family member 9B (TBC1D9B) a Rab11A GTPase-activating protein (Rab11A GAP), as a LMTK1 binding protein, and TBC1D9B inactivates Rab11A under the control of LMTK1A (PMID: 31628178). TBC1D9B is associated with endocytic vesicles, including those likely to contain active Rab11a (PMID: 25232007). TBC1D9B could also interact with other mammalian ATG8 homologues. TBC1D9B could be co-localized with LC3B on the autophagosome membranes (PMID: 30202024). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14019 Product name: Recombinant human TBC1D9B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1069-1250 aa of BC000701 Sequence: ARTGRKPRDCATEEDEPPAPELHQDAARELQPPAAGDPQAKAGGDTHLGTAPQESQVVVEGGSGEGQGSPSQLLSDDETKDDMSMSSYSVVSTGSLQCEDLADDTVLVGGEACSPTARIGGTVDTDWCISFEQILASILTESVLVNFFEKRVDIGLKIKDQKKVERQFSTASDHEQPGVSG Predict reactive species Full Name: TBC1 domain family, member 9B (with GRAM domain) Calculated Molecular Weight: 1250 aa, 140 kDa Observed Molecular Weight: 141-150 kDa GenBank Accession Number: BC000701 Gene Symbol: TBC1D9B Gene ID (NCBI): 23061 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q66K14 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.