Proteintech, 20182-1-AP, FSTL1 Polyclonal antibody

Size: 20ul / 150ul The FSTL1 (20182-1-AP) by Proteintech is a Polyclonal antibody targeting FSTL1 in WB, IHC, IP, ELISA applications with reactivity to human, rat samples 20182-1-AP targets FSTL1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: A2780 cells, HeLa cells, human placenta tissue, A431 cells, C6 cells Positive IP detected in: A2780 cells Positive IHC detected in: human colon tissue, human placenta tissue, rat colon tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Follistatin-like 1 (FSTL1), initially discovered as a TGF-β1-induced gene, encodes a 308 amino acid secreted 45-55 kDa glycoprotein with a follistatin domain and two non-functional calcium-binding motifs. FSTL1 has been reported to be involved in the fate determination and maturation of epithelial cells. Ablation of the FSTL1 gene in the mouse results in several structural developmental defects and neonatal lethality due to respiratory failure, demonstrating its functional importance. FSTL1 has been reported to exhibit both pro- and anti-inflammatory actions, with a specific anti-apoptotic and protective effect in cardiac or renal cell injury. Zwijsen et al. (1994) detected several isoforms of FSTL1 with molecular masses of 40 to 48 kD which differs from the 50- to 55-kD products detected by Tanaka et al. (1998) (OMIM). (PMID: 26386648; PMID: 23723173; PMID: 20176958). Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14057 Product name: Recombinant human FSTL1 protein Source: e coli. -derived, PKG Tag: GST Domain: 1-308 aa of BC000055 Sequence: MWKRWLALALALVAVAWVRAEEELRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSVSPSASPVVCYQSNRDELRRRIIQWLEAEIIPDGWFSKGSNYSEILDKYFKNFDNGDSRLDSSEFLKFVEQNETAINITTYPDQENNKLLRGLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCACGNWVCTAMTCDGKNQKGAQTQTEEEMTRYVQELQKHQETAEKTKRVSTKEI Predict reactive species Full Name: follistatin-like 1 Calculated Molecular Weight: 308 aa, 35 kDa Observed Molecular Weight: 35-40 kDa GenBank Accession Number: BC000055 Gene Symbol: FSTL1 Gene ID (NCBI): 11167 RRID: AB_10666439 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q12841 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.