Proteintech, 20257-1-AP, SCN9A/Nav1.7-Specific Polyclonal antibody

Size: 20ul / 150ul The SCN9A/Nav1 20257-1-AP targets SCN9A/Nav1.7-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, pig brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information SCN9A, also named as NENA, PN1, ETHA, NE-NA, Nav1.7 and hNE-Na, belongs to the sodium channel family. SCN9A mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, SCN9A forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na+ channel isoform. SCN9a plays a role in pain mechanisms, especially in the development of inflammatory pain. Defects in SCN9A are the cause of primary erythermalgia or autosomal recessive congenital indifference to pain or paroxysmal extreme pain disorder (PEPD). The antibody is specific to SCN9A Specification Tested Reactivity: human, mouse, pig Cited Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: sodium channel, voltage-gated, type IX, alpha subunit Calculated Molecular Weight: 226 kDa Observed Molecular Weight: 226 kDa GenBank Accession Number: NM_002977 Gene Symbol: SCN9A Gene ID (NCBI): 6335 RRID: AB_11182704 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15858 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.