Proteintech, 20260-1-AP, TAF1-Specific Polyclonal antibody

Size: 20ul / 150ul The TAF1-Specific (20260-1-AP) by Proteintech is a Polyclonal antibody targeting TAF1-Specific in WB, IHC, ELISA applications with reactivity to human, mouse samples 20260-1-AP targets TAF1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, mouse brain tissue, HeLa cells, Jurkat cells, K-562 cells Positive IHC detected in: human prostate cancer tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Background Information TAF1, also named as BA2R, CCG1, CCGS, TAF2A, p250, Cell cycle gene 1 protein, TAFII250 and DYT3, belongs to the TAF1 family. TAF1 is the largest component and core scaffold of the TFIID basal transcription factor complex. It contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. TAF1 phosphorylates GTF2A1 and GTF2F1 on Ser residues. It possesses DNA-binding activity. It is essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3) which also called X-linked dystonia-parkinsonism (XDP). The antibody is specific to TAF1. It has several isoforms with predicted MW's of 200-250 kDa and 170-180 kDa, it also could get about 50 kDa band which is caused by the lysate preparation. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa Calculated Molecular Weight: 213 kDa Observed Molecular Weight: 250-260 kDa, 50 kDa GenBank Accession Number: NM_138923 Gene Symbol: TAF1 Gene ID (NCBI): 6872 RRID: AB_10693532 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P21675 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.