Proteintech, 20280-1-AP, RREB1-Specific Polyclonal antibody

Size: 20ul / 150ul The RREB1-Specific (20280-1-AP) by Proteintech is a Polyclonal antibody targeting RREB1-Specific in WB, IHC, ELISA applications with reactivity to human samples 20280-1-AP targets RREB1-Specific in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: BxPC-3 cells, SH-SY5Y cells, HeLa cells Positive IHC detected in: human stomach tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information RREB1, also named as FINB and Zep-1, belongs to the krueppel C2H2-type zinc-finger protein family. RREB1 is a transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. It may be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. RREB1 represses the angiotensinogen gene. RREB1 can induce expression of p53 and represse p16 which associated with the expression of tumour. It has been reported that the RREB1 can be a candidate gene for type 2 diabetes associated end-stage kidney disease. To some extent, the decrease of zinc in human pancreatic adenocarcinoma is associated with the downregulation of RREB1. RREB1 has some isoforms with 250 kDa, 180-200 kDa and 140-160 kDa protein.(PMID: 21703425, 15067362, 25050557, 25027322) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ras responsive element binding protein 1 Calculated Molecular Weight: 181 kDa Observed Molecular Weight: 160-181 kDa, 250 kDa GenBank Accession Number: NM_001003698 Gene Symbol: RREB1 Gene ID (NCBI): 6239 RRID: AB_2878661 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92766 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.