Proteintech, 20355-1-AP, MCT2/SLC16A7 Polyclonal antibody

Size: 20ul / 150ul The MCT2/SLC16A7 (20355-1-AP) by Proteintech is a Polyclonal antibody targeting MCT2/SLC16A7 in WB, IHC, ELISA applications with reactivity to human, mouse samples 20355-1-AP targets MCT2/SLC16A7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: SH-SY5Y cells, HEK-293 cells, K-562 cells Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information SLC16A7, also named MCT2, is a proton-coupled monocarboxylate transporter catalyzing the rapid transport across the plasma membrane of many monocarboxylates. It has been reported that SLC16A7 is the primary monocarboxylate transporter in Purkinje cells and can be detected in the brain, cerebellum, liver, and kidney, but not in vascular smooth muscle cells, pericytes, endothelium, heart, lung, or stomach. There are some isoforms of SLC16A7 with bands around 52 kDa and 40-45 kDa in SDS-PAGE. The 80 kDa protein might be the dimer similar to the papers published. (PMID: 9482213, 15749979, 11291733, 16901736) Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14201 Product name: Recombinant human SLC16A7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 433-478 aa of BC030693 Sequence: AKERKEENARQKTRESEPLSKSKHSEDVNVKVSNAQSVTSERETNI Predict reactive species Full Name: solute carrier family 16, member 7 (monocarboxylic acid transporter 2) Calculated Molecular Weight: 478 aa, 52 kDa Observed Molecular Weight: 40-52 kDa GenBank Accession Number: BC030693 Gene Symbol: MCT2 Gene ID (NCBI): 9194 RRID: AB_2878680 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60669 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.