Proteintech, 20371-1-AP, SFRS2 Polyclonal antibody

Size: 20ul / 150ul The SFRS2 (20371-1-AP) by Proteintech is a Polyclonal antibody targeting SFRS2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 20371-1-AP targets SFRS2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: NIH/3T3 cells, A549 cells, mouse testis tissue, rat brain tissue, Calu-1 cells, HepG2 cells, L02 cells Positive IP detected in: mouse brain tissue Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:1500-1:6000 Background Information SFRS2, also named as PR264 and Splicing component, 35 kDa, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. Some residue of SFRS2 is modified by phosphorylase and acetylase after translation. The molecular weight of modified SFRS2 is 35-40kDa. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. The antibody has no cross reaction to SFRS2B. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: splicing factor, arginine/serine-rich 2 Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 25-35 kDa GenBank Accession Number: NM_003016 Gene Symbol: SFRS2 Gene ID (NCBI): 6427 RRID: AB_2616311 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q01130 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.