Proteintech, 20681-1-AP, ABCA2 Polyclonal antibody

Size: 20ul / 150ul The ABCA2 (20681-1-AP) by Proteintech is a Polyclonal antibody targeting ABCA2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 20681-1-AP targets ABCA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse thymus tissue, HepG2 cells, L02 cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information ABCA2, also named as ABC2 and KIAA1062, belongs to the ABC transporter ABCA2, a member of the ATP-binding cassette (ABC) transporter family, is primarily expressed in brain and is involved in brain sterol homeostasis and associated with early onset Alzheimer's disease. ABCA2 is mainly localized in endosomes and lysosomes of oligodendrocytes and Schwann cells. It has also be reported as a marker of neural progenitor in the adult rodent brain. (PMID: 16539677) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ATP-binding cassette, sub-family A (ABC1), member 2 Calculated Molecular Weight: 270 kDa Observed Molecular Weight: 270 kDa GenBank Accession Number: NM_001606 Gene Symbol: ABCA2 Gene ID (NCBI): 20 RRID: AB_10859780 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BZC7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.