Proteintech, 20683-1-AP, DOCK3; MOCA Polyclonal antibody

Size: 20ul / 150ul The DOCK3; MOCA (20683-1-AP) by Proteintech is a Polyclonal antibody targeting DOCK3; MOCA in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 20683-1-AP targets DOCK3; MOCA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, human brain tissue, SH-SY5Y cells, rat brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Dedicator of cytokinesis 3 (DOCK3), also named as MOCA and PBP , is a ~180 kDa protein involved in signaling trasduction. It is a potential guanine nucleotide exchange factor (GEF) which activate some small GTPases by exchanging bound GDP for free GTP. DOCK3 is associated in Alzheimer disease tangles and regulates the accumulation of amyloid precursor protein and beta-amyloid. Overexpression of Dock3 in neural cells promotes axonal outgrowth downstream of brain-derived neurotrophic factor (BDNF) signaling. DOCK3 binds to and inactivates glycogen synthase kinase-3β (GSK-3β) at the plasma membrane, thereby promoteing axon branching and microtubule assembly. By stimulating actin polymerization and microtubule assembly, DOCK3 plays important roles downstream of BDNF signaling in the CNS. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: dedicator of cytokinesis 3 Calculated Molecular Weight: 233 kDa Observed Molecular Weight: 233 kDa GenBank Accession Number: NM_004947 Gene Symbol: DOCK3 Gene ID (NCBI): 1795 RRID: AB_10696182 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IZD9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.