Proteintech, 20689-1-AP, RELN Polyclonal antibody

Size: 20ul / 150ul The RELN (20689-1-AP) by Proteintech is a Polyclonal antibody targeting RELN in IHC, IF-P, ELISA applications with reactivity to human, mouse samples 20689-1-AP targets RELN in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IHC detected in: human liver cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information RELN belongs to the reelin family. It is an extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. It is a secreted glycoprotein which is essential for neuronal migration in the cortex. RELN regulates microtubule function in neurons and neuronal migration. It affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and ApoER2, RELN induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. Defects in RELN are the cause of lissencephaly type 2 (LIS2). Specification Tested Reactivity: human, mouse Cited Reactivity: mouse, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: reelin Calculated Molecular Weight: 388 kDa GenBank Accession Number: NM_005045 Gene Symbol: RELN Gene ID (NCBI): 5649 RRID: AB_2878724 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P78509 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.