Proteintech, 20700-1-AP, RUNX2 Polyclonal antibody

Size: 20ul / 150ul The RUNX2 (20700-1-AP) by Proteintech is a Polyclonal antibody targeting RUNX2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, rat samples 20700-1-AP targets RUNX2 in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: ROS1728 cells, MCF-7 cells Positive IP detected in: ROS1728 cells Positive IHC detected in: rat skin tissue, rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: ROS1728 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2. Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse, rat, pig, rabbit, chicken Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: runt-related transcription factor 2 Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 57-60 kDa GenBank Accession Number: NM_004348 Gene Symbol: RUNX2 Gene ID (NCBI): 860 RRID: AB_2722783 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13950 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.