Proteintech, 20717-1-AP, ESPN Polyclonal antibody

Size: 20ul / 150ul The ESPN (20717-1-AP) by Proteintech is a Polyclonal antibody targeting ESPN in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 20717-1-AP targets ESPN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, Neuro-2a cells, rat testis Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:300-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ESPN, also named as DFNB36, is a multifunctional actin-bundling protein. ESPN plays a major role in regulating the organization, dimensions, dynamics and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in variouS mechanosensory and chemosensory cells. Defects in ESPN are the cause of deafness autosomal recessive type 36 (DFNB36). Defects in ESPN are the cause of deafness autosomal dominant without vestibular involvement (DFNAWVI). The antibody is specific to ESPN. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: espin Calculated Molecular Weight: 92 kDa Observed Molecular Weight: 30-33 kDa, 110 kDa GenBank Accession Number: NM_031475 Gene Symbol: ESPN Gene ID (NCBI): 83715 RRID: AB_10858327 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: B1AK53 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.