Proteintech, 20735-1-AP, SYNM Polyclonal antibody

Size: 20ul / 150ul The SYNM (20735-1-AP) by Proteintech is a Polyclonal antibody targeting SYNM in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 20735-1-AP targets SYNM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Y79 cells, human skeletal muscle tissue Positive IHC detected in: human skeletal muscle tissue, human bladder tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information SYNM, also named as DMN, KIAA0353 and SYN, belongs to the intermediate filament family. SYNM is a Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells. The antibody can't recognize isoform 2 or 3 of SYNM. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: synemin, intermediate filament protein Calculated Molecular Weight: 173 kDa Observed Molecular Weight: 170-180 kDa GenBank Accession Number: NM_015286 Gene Symbol: SYNM Gene ID (NCBI): 23336 RRID: AB_11042328 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O15061 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.