Proteintech, 20792-1-AP, RNF133 Polyclonal antibody

Size: 20ul / 150ul The RNF133 (20792-1-AP) by Proteintech is a Polyclonal antibody targeting RNF133 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 20792-1-AP targets RNF133 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, MCF-7 cells Positive IHC detected in: mouse testis tissue, human brain tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information RNF133 (ring finger protein 133) contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14537 Product name: Recombinant human RNF133 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 208-376 aa of BC022038 Sequence: YHIHRLCLARIQNRRWQRLTTDLQNTFGQLQLRVVKEGDEEINPNGDSCVICFERYKPNDIVRILTCKHFFHKNCIDPWILPHGTCPICKCDILKVLGIQVVVENGTEPLQVLMSNELPETLSPSEEETNNEVSPAGTSDKVIHVEENPTSQNNDIQPHSVVEDVHPSP Predict reactive species Full Name: ring finger protein 133 Calculated Molecular Weight: 376 aa, 42 kDa Observed Molecular Weight: 45-48 kDa GenBank Accession Number: BC022038 Gene Symbol: RNF133 Gene ID (NCBI): 168433 RRID: AB_10695189 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WVZ7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.