Proteintech, 20805-1-AP, HTATSF1 Polyclonal antibody

Size: 20ul / 150ul The HTATSF1 (20805-1-AP) by Proteintech is a Polyclonal antibody targeting HTATSF1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 20805-1-AP targets HTATSF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse brain tissue, HepG2 cells, rat brain tissue, Jurkat cells Positive IP detected in: HepG2 cells Positive IHC detected in: human breast cancer tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Hela cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14744 Product name: Recombinant human HTATSF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC009896 Sequence: MSGTNLDGNDEFDEQLRMQELYGDGKDGDTQTDAGGEPDSLGQQPTDTPYEWDLDKKAWFPKITEDFIATYQANYGFSNDGASSSTANVEDVHARTAEEPPQEKAPEPTDARKKGEKRKAESGWFHVEEDRNTNVYVSGLPPDITVDEFIQLMSKFGIIMRDPQTEEFKVKLYKDNQGNLKGDGLCCYLKRESVELALKLLDEDEIRGYKLHVEVAKFQLKGEYDASKKKKKCKDYKKKLSMQQKQLDWRPERRAGPSRMRHERVVIIKNMFHPMDFEDDPLVLNEIREDLRVECSKFGQIRKLLLFDRHPDGVASVSFRDPEEADYCIQTLDGRWFGGRQITAQAWDGT Predict reactive species Full Name: HIV-1 Tat specific factor 1 Calculated Molecular Weight: 755 aa, 86 kDa Observed Molecular Weight: 130-140 kDa GenBank Accession Number: BC009896 Gene Symbol: HTATSF1 Gene ID (NCBI): 27336 RRID: AB_10695767 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43719 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.