Proteintech, 20825-1-AP, SGSM3 Polyclonal antibody

Size: 20ul / 150ul The SGSM3 (20825-1-AP) by Proteintech is a Polyclonal antibody targeting SGSM3 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 20825-1-AP targets SGSM3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: fetal human brain tissue, L02 cells, mouse brain tissue, mouse testis tissue, rat testis tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human heart tissue, human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Small G protein signaling modulator 3 (SGSM3) is also named as MAP, RABGAPLP and RABGAPLP. SGSM3 is highly correlated with Cx43 in heart functions and that high levels of SGSM3 may induce Cx43 turnover through lysosomal degradation in infarcted rat hearts (PMID: 29577900, PMID: 32271805). SGSM3 has been shown to be associated with small G-protein-coupled receptor signaling (PMID: 29693742). Several studies have shown that SGSM3 is associated with the risk of some cancers, including liver, breast, colorectal and bladder cancers (PMID: 32271805). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14800 Product name: Recombinant human SGSM3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 400-749 aa of BC008078 Sequence: RTQRRKSTITALLFGEDDLEALKAKNIKQTELVADLREAILRVARHFQCTDPKNCSVELTPDYSMESHQRDHENYVACSRSHRRRAKALLDFERHDDDELGFRKNDIITIVSQKDEHCWVGELNGLRGWFPAKFVEVLDERSKEYSIAGDDSVTEGVTDLVRGTLCPALKALFEHGLKKPSLLGGACHPWLFIEEAAGREVERDFASVYSRLVLCKTFRLDEDGKVLTPEELLYRAVQSVNVTHDAVHAQMDVKLRSLICVGLNEQVLHLWLEVLCSSLPTVEKWYQPWSFLRSPGWVQIKCELRVLCCFAFSLSQDWELPAKREAQQPLKEGVRDMLVKHHLFSWDVDG Predict reactive species Full Name: small G protein signaling modulator 3 Calculated Molecular Weight: 749 aa, 85 kDa Observed Molecular Weight: 85 kDa GenBank Accession Number: BC008078 Gene Symbol: SGSM3 Gene ID (NCBI): 27352 RRID: AB_10693680 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96HU1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.