Proteintech, 20848-1-AP, C9orf86 Polyclonal antibody

Size: 20ul / 150ul The C9orf86 (20848-1-AP) by Proteintech is a Polyclonal antibody targeting C9orf86 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 20848-1-AP targets C9orf86 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MDA-MB-453s cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information C9orf86, also known as RBEL1 (Rab-like protein 1), is a novel subfamily of GTPases within the Ras superfamily. It has two splice variants, RBEL1Aand RBEL1B. Unlike known Rabs that aremostly cytosolic, RBEL1B predominantly resides in the nucleus, whereas RBEL1A is localized primarily to the cytosol. C9orf86 is overexpressed in the majority of primary breast tumors, and knockdown of C9orf86 in MCF-7 breast cancer cells resulted in cell growth suppression associated with apoptosis [PMID: 23977139]. Western blot analysis revealed that the endogenous RBEL1 migrated at 110-125 kDa, 80 kDa, maybe due to a number of potential O-linked glycosylation sites.[PMID: 17962191] Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14350 Product name: Recombinant human C9orf86 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-309 aa of BC002945 Sequence: MFSALKKLVGSDQAPGRDKNIPAGLQSMNQALQRRFAKGVQYNMKIVIRGDRNTGKTALWHRLQGRPFVEEYIPTQEIQVTSIHWSYKTTDDIVKVEVWDVVDKGKCKKRGDGLKMENDPQEAESEMALDAEFLDVYKNCNGVVMMFDITKQWTFNYILRELPKVPTHVPVCVLGNYRDMGEHRVILPDDVRDFIDNLDRPPGSSYFRYAESSMKNSFGLKYLHKFFNIPFLQLQRETLLRQLETNQLDMDATLEELSVQQETEDQNYGIFLEMMEARSRGHASPLAANGQSPSPGSQSPVVPAGAVST Predict reactive species Full Name: chromosome 9 open reading frame 86 Calculated Molecular Weight: 729 aa, 80 kDa Observed Molecular Weight: 125 kDa GenBank Accession Number: BC002945 Gene Symbol: C9orf86 Gene ID (NCBI): 55684 RRID: AB_2878750 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q3YEC7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.