Proteintech, 20882-1-AP, CUL4B Polyclonal antibody

Size: 20ul / 150ul The CUL4B (20882-1-AP) by Proteintech is a Polyclonal antibody targeting CUL4B in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 20882-1-AP targets CUL4B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse thymus tissue, Jurkat cells, Y79 cells, SH-SY5Y cells, HeLa cells, rat thymus tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human tonsillitis tissue, human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). Proteintech's CUL4B antibody 20882-1-AP can specifically recognize CUL4B. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15024 Product name: Recombinant human CUL4B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-195 aa of BC036216 Sequence: MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAK Predict reactive species Full Name: cullin 4B Calculated Molecular Weight: 913 aa, 104 kDa Observed Molecular Weight: 102 kDa GenBank Accession Number: BC036216 Gene Symbol: CUL4B Gene ID (NCBI): 8450 RRID: AB_10694831 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13620 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.