Proteintech, 20906-1-AP, C3orf20 Polyclonal antibody

Size: 20ul / 150ul The C3orf20 (20906-1-AP) by Proteintech is a Polyclonal antibody targeting C3orf20 in IHC, IP, ELISA applications with reactivity to human, mouse samples 20906-1-AP targets C3orf20 in IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: SK-N-SH cells, human brain tissue, mouse brain tissue, mouse testis tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The function of C3orf20 has not been widely studied, and is yet to be fully elucidated. Two isoforms were produced by alternative splicing with predicted MW of 88 and 101 kDa according to UniProt. Catalog#20906-1-AP can recognize both 88 and 101 kDa isoforms. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15031 Product name: Recombinant human C3orf20 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 555-904 aa of BC038406 Sequence: FINSILLAAGLFTIEYPTKKEEEEFVRFKMRSRTHPERLPKLSLYSGESLLRSQSGHLESSIAETLKDEPESAPVSPVRKTTKIHTKAKVTSRGKAREGRSPTRWAALPSDCPLVLRKLMLKEDTRAGCKCLVKAPLVSDVELERFLLAPRDPSQVLVFGIISSQNYTSTGQLQWLLNTLYNHQQRGRGSPCIQCRYDSYRLLQYDLDSPLQEDPPLMVKKNSVVQGMILMFAGGKLIFGGRVLNGYGLSKQNLLKQIFRSQQDYKMGYFLPDDYKFSVPNSVLSLEDSESVKKAESEDIQGSSSSLALEDYVEKELSLEAEKTREPEVELHPLSRDSKITSWKKQASKK Predict reactive species Full Name: chromosome 3 open reading frame 20 Calculated Molecular Weight: 904 aa, 101 kDa Observed Molecular Weight: 101 kDa, 88 kDa GenBank Accession Number: BC038406 Gene Symbol: C3orf20 Gene ID (NCBI): 84077 RRID: AB_10732599 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8ND61 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.