Proteintech, 20960-1-AP, HIF-1 alpha Polyclonal antibody

Size: 20ul / 150ul The HIF-1 alpha (20960-1-AP) by Proteintech is a Polyclonal antibody targeting HIF-1 alpha in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 20960-1-AP targets HIF-1 alpha in WB, IHC, IF/ICC, IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Cobalt Chloride treated HeLa cells Positive IP detected in: HeLa cells Positive IHC detected in: human thyroid cancer tissue, human kidney tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). . Specification Tested Reactivity: human Cited Reactivity: human, pig, chicken, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15198 Product name: Recombinant human HIF1a protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 574-799 aa of BC012527 Sequence: LRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYD Predict reactive species Full Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) Calculated Molecular Weight: 826 aa, 93 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC012527 Gene Symbol: HIF1A Gene ID (NCBI): 3091 RRID: AB_10732601 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q16665 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.