Proteintech, 20968-1-AP, CYP11B2 Polyclonal antibody

Size: 20ul / 150ul The CYP11B2 (20968-1-AP) by Proteintech is a Polyclonal antibody targeting CYP11B2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, pig samples 20968-1-AP targets CYP11B2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples. Tested Applications Positive WB detected in: SKOV-3 cells, pig adrenal gland tissue Positive IHC detected in: human adrenal gland tissue, human kidney tissue, human hepatocirrhosis tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: SKOV-3 cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information CYP11B2 (Cytochrome P450 11B2), also known as aldosterone synthase, is a member of the cytochrome P450 superfamily of enzymes. CYP11B2 can only catalyze 11-deoxycorticosterone oxidation to produce corticosterone and further hydroxylation to ALD. CYP11B2 is an enzyme required for cortisol biosynthesis and is specifically expressed in aldosterone-producing tissue of the adrenal cortex and is overexpressed in aldosterone-producing adenomas (APA). Specification Tested Reactivity: human, mouse, pig Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cytochrome P450, family 11, subfamily B, polypeptide 2 Calculated Molecular Weight: 58 kDa Observed Molecular Weight: 48-50 kDa GenBank Accession Number: NM_000498 Gene Symbol: CYP11B2 Gene ID (NCBI): 1585 RRID: AB_2878777 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P19099 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.