Proteintech, 20977-1-AP, RAG1 Polyclonal antibody

Size: 20ul / 150ul The RAG1 (20977-1-AP) by Proteintech is a Polyclonal antibody targeting RAG1 in WB, ELISA applications with reactivity to human, mouse, rat samples 20977-1-AP targets RAG1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse thymus tissue, rat thymus tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Recombination-activating gene (RAG) proteins include RAG1 and RAG2, which are crucial for Ig and T lymphocyte receptor (TCR) fragment rearrangement and are responsible for DNA recognition and cleavage at specific sequences. Mutations in RAG1 result in the complete or partial loss of recombinant enzyme activity, in unbalanced variable-diversity-joining (V(D)J) recombination, and in the impairment of T and B lymphocyte development at the early stages. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: recombination activating gene 1 Calculated Molecular Weight: 119 kDa Observed Molecular Weight: 110-120 kDa GenBank Accession Number: NM_000448 Gene Symbol: RAG1 Gene ID (NCBI): 5896 RRID: AB_3669352 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P15918 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.