Proteintech, 21021-1-AP, PDXDC1 Polyclonal antibody

Size: 20ul / 150ul The PDXDC1 (21021-1-AP) by Proteintech is a Polyclonal antibody targeting PDXDC1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 21021-1-AP targets PDXDC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse testis tissue, HeLa cells, SGC-7901 cells, rat testis tissue Positive IP detected in: HepG2 cells Positive IHC detected in: human hepatocirrhosis tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PDXDC1 (pyridoxal-dependent decarboxylase domain containing 1) is a putative enzyme that could metabolize catecholamine neurotransmitters (PMID:28485732). With prior evidence for involvement with glioblastoma from other previously reported experimental settings, and contains the lead single nucleotide polymorphism (rs3198697) from the linkage analysis of the chromosome 16 region (PMID:32644145). Moreover, PDXDC1 is involved in the catalysis of the nonhydrolytic addition or removal of a carboxyl group to or from a compound (PMID:29277971). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15283 Product name: Recombinant human PDXDC1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC033748 Sequence: MDASLEKIADPTLAEMGKNLKEAVKMLEDSQRRTEEENGKKLISGDIPGPLQGSGQDMVSILQLVQNLMHGDEDEEPQSPRIQNIGEQGHMALLGHSLGAYISTLDKEKLRKLTTRILSDTTLWLCRIFRYENGCAYFHEEEREGLAKICRLAIHSRYEDFVVDGFNVLYNKKPVIYLSAAARPGLGQYLCNQDVAFLEKLIKDDIERGRLPLLLVANAGTAAVGHTDKIGRLKELCEQYGIWLHVEGVNLATLALGYVSSSVLAAAKCDSMTMTPGPWLGLPAVPAVTLYKHDDPALTLVAGLTSNKPTDKLRALPLWLSLQYLGLDGFVERIKHACQLSQRLQ Predict reactive species Full Name: pyridoxal-dependent decarboxylase domain containing 1 Calculated Molecular Weight: 788 aa, 87 kDa Observed Molecular Weight: 87 kDa GenBank Accession Number: BC033748 Gene Symbol: PDXDC1 Gene ID (NCBI): 23042 RRID: AB_10695768 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6P996 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.