Proteintech, 21082-1-AP, CCDC50 Polyclonal antibody

Size: 20ul / 150ul The CCDC50 (21082-1-AP) by Proteintech is a Polyclonal antibody targeting CCDC50 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 21082-1-AP targets CCDC50 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, L02 cells, mouse lung tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue Positive IHC detected in: human kidney tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CCDC50 encodes Ymer, an effector of epidermal growth factor (EGF)-mediated cell signaling that is ubiquitously expressed in different organs and has been suggested to inhibit down-regulation of the EGF receptor. CCDC50 act as a multifunctional regulator in NF-κB and Fas signaling pathways. In chronic lymphocytic leukaemia (CLL) and mantle cell lymphoma (MCL), CCDC50 is required for the survival of MCL and CLL cancer cells. Recently, CCDC50 was shown to promote hepatocellular carcinoma through the Ras/Foxo4 signaling pathway. (PMID: 34453126, PMID: 32612200, PMID: 17503326) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15293 Product name: Recombinant human CCDC50 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC065004 Sequence: MAEVSIDQSKLPGVKEVCRDFAVLEDHTLAHSLQEQEIEHHLASNVQRNRLVQHDLQVAKQLQEEDLKAQAQLQKRYKDLEQQDCEIAQEIQEKLAIEAERRRIQEKKDEDIARLLQEKELQEEKKRKKHFPEFPATRAYADSYYYEDGGMKPRVMKEAVSTPSRMAHRDQEWYDAEIARKLQEEELLATQVDMRAAQVAQDEEIARLLMAEEKKAYKKAKEREKSSLDKRKQDPEWKPKTAKAANSKSKESDEPHHSKNERPARPPPPIMTDGEDADYTHFTNQQSSTRHFSKSESSHKGFHYKH Predict reactive species Full Name: coiled-coil domain containing 50 Calculated Molecular Weight: 482 aa, 56 kDa Observed Molecular Weight: 36-38 kDa GenBank Accession Number: BC065004 Gene Symbol: CCDC50 Gene ID (NCBI): 152137 RRID: AB_10695770 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IVM0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.