Proteintech, 21200-1-AP, PDE6A Polyclonal antibody

Size: 20ul / 150ul The PDE6A (21200-1-AP) by Proteintech is a Polyclonal antibody targeting PDE6A in WB, IHC, IF, ELISA applications with reactivity to human, mouse, rat samples 21200-1-AP targets PDE6A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse eye tissue, human brain tissue, rat eye tissue Positive IHC detected in: mouse eye tissue, human colon cancer tissue, human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF detected in: retinal tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF): IF : 1:50-1:200 Background Information PDE6A(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha) is also named as GMP-PDE alpha, PDE V-B1, PDEA and belongs to the cyclic nucleotide phosphodiesterase family. PDE6A contains an open reading frame capable of coding for a polypeptide of 859 amino acids and about 100 kDa. It is a subunit of a key phototransduction enzyme which participates in processes of transmission and amplification of the visual signal. The expression of PDE6 alpha in retina is essential for normal expression of PDE6 beta and PDE6 gama. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15562 Product name: Recombinant human PDE6A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-90 aa of BC035909 Sequence: MGEVTAEEVEKFLDSNIGFAKQYYNLHYRAKLISDLLGAKEAAVDFSNYHSPSSMEESEIIFDLLRDFQENLQTEKCIFNVMKKLCFLLQ Predict reactive species Full Name: phosphodiesterase 6A, cGMP-specific, rod, alpha Calculated Molecular Weight: 860 aa, 100 kDa Observed Molecular Weight: 100-110 kDa GenBank Accession Number: BC035909 Gene Symbol: PDE6A Gene ID (NCBI): 5145 RRID: AB_10733345 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P16499 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.