Proteintech, 21205-1-AP, NUDCD2 Polyclonal antibody

Size: 20ul / 150ul The NUDCD2 (21205-1-AP) by Proteintech is a Polyclonal antibody targeting NUDCD2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 21205-1-AP targets NUDCD2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, HEK-293 cells, human brain tissue, human kidney tissue, mouse brain tissue, rat brain tissue Positive IP detected in: HepG2 cells Positive IHC detected in: human kidney tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15579 Product name: Recombinant human NUDCD2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-157 aa of BC017934 Sequence: MSAPFEERSGVVPCGTPWGQWYQTLEEVFIEVQVPPGTRAQDIQCGLQSRHVALSVGGREILKGKLFDSTIADEGTWTLEDRKMVRIVLTKTKRDAANCWTSLLESEYAADPWVQDQMQRKLTLERFQKENPGFDFSGAEISGNYTKGGPDFSNLEK Predict reactive species Full Name: NudC domain containing 2 Calculated Molecular Weight: 157 aa, 18 kDa Observed Molecular Weight: 18 kDa GenBank Accession Number: BC017934 Gene Symbol: NUDCD2 Gene ID (NCBI): 134492 RRID: AB_10732725 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WVJ2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.