Proteintech, 21214-1-AP, PLOD2 Polyclonal antibody

Size: 20ul / 150ul The PLOD2 (21214-1-AP) by Proteintech is a Polyclonal antibody targeting PLOD2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 21214-1-AP targets PLOD2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Caco-2 cells, A431 cells, DU 145 cells, HT-1080 cells, THLE-2 cells, HEK-293 cells, HeLa cells, HepG2 cells Positive IP detected in: mouse testis tissue Positive IHC detected in: human liver cancer tissue, human pancreas tissue, human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rabbit, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15614 Product name: Recombinant human PLOD2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 459-540 aa of BC037169 Sequence: PYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQMLSPPKGVFMYISNRHEFGRLLSTA Predict reactive species Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 Calculated Molecular Weight: 758 aa, 85 kDa Observed Molecular Weight: 87 kDa GenBank Accession Number: BC037169 Gene Symbol: PLOD2 Gene ID (NCBI): 5352 RRID: AB_10733347 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O00469 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.