Proteintech, 21302-1-AP, JAML Polyclonal antibody

Size: 20ul / 150ul The JAML (21302-1-AP) by Proteintech is a Polyclonal antibody targeting JAML in WB, IHC, ELISA applications with reactivity to human samples 21302-1-AP targets JAML in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HL-60 cells, Jurkat cells, L02 cells Positive IHC detected in: human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Junctional adhesion molecules (JAM-A, JAM-B, and JAM-C) are endothelial and epithelial adhesion molecules involved in the recruitment of circulating leukocytes to inflammatory sites (PMID: 19064666). JAML (junctional adhesion molecule-like), also known as AMICA1, is related to JAM proteins. It contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail. JAML is expressed on leukocytes and promotes their adhesion to endothelial cells (PMID: 12869515). JAML can regulate neutrophil transepithelial migration via binding interactions with epithelial coxsackie and adenovirus receptor (CAR) (PMID: 15800062). The full-length human JAML protein has a calculated molecular weight of 44 kDa. This antibody raised against 40-275 aa of human JAML protein detects bands with molecular wight of 44 kDa, and 50-70 kDa which probably represent different glycoforms of the protein (PMID: 12869515; 24621992; 15800062). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15900 Product name: Recombinant human AMICA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 40-275 aa of BC100797 Sequence: VPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVVLHVLPEEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCSIHLGNLVFKKTIVLHVSPEEPRTLVTPAALRPLVLGGNQLVIIVGIVCATILLLPVLILIVKKTCGNKSSVNSTVLVKN Predict reactive species Full Name: adhesion molecule, interacts with CXADR antigen 1 Calculated Molecular Weight: 394 aa, 44 kDa Observed Molecular Weight: 44 kDa, 50-70 kDa GenBank Accession Number: BC100797 Gene Symbol: JAML Gene ID (NCBI): 120425 RRID: AB_10733103 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86YT9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.