Proteintech, 21480-1-AP, SYNPO2L Polyclonal antibody

Size: 20ul / 150ul The SYNPO2L (21480-1-AP) by Proteintech is a Polyclonal antibody targeting SYNPO2L in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 21480-1-AP targets SYNPO2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, PC-3 cells, rat testis tissue Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SYNPO2L belongs to the synaptopodin family and is an actin-associated protein that may play a role in modulating actin-based shape. SYNPO2L is involved in the positive regulation of Rho protein signal transduction and the positive regulation of stress fiber assembly. Loss-of-function variants in the SYNPO2L gene are associated with atrial fibrillation (PMID: 33768119). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15683 Product name: Recombinant human SYNPO2L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 35-389 aa of BC142635 Sequence: LQRAESLQEKSIKEAKTKCRTIASLLTAAPNPHSKGVLMFKKRRQRAKKYTLVSFGAAAGTGAEEEDGVPPTSESELDEEAFSDARSLTNQSDWDSPYLDMELARAGSRASEGQGSGLGGQLSEVSGRGVQLFEQQRQRADSSTQELARVEPAAMLNGEGLQSPPRAQSAPPEAAVLPPSPLPAPVASPRPFQPGGGAPTPAPSIFNRSARPFTPGLQGQRPTTTSVIFRPLAPKRANDSLGGLSPAPPPFLSSQGPTPLPSFTSGVPSHAPVSGSPSTPRSSGPVTATSSLYIPAPSRPVTPGGAPEPPAPPSAAAMTSTASIFLSAPLRPSARPEAPAPGPGAPEPPSAREQR Predict reactive species Full Name: synaptopodin 2-like Calculated Molecular Weight: 977 aa, 102 kDa Observed Molecular Weight: 79 kDa GenBank Accession Number: BC142635 Gene Symbol: SYNPO2L Gene ID (NCBI): 79933 RRID: AB_10860260 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H987 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.