Proteintech, 21832-1-AP, ZP2 Polyclonal antibody

Size: 20ul / 150ul The ZP2 (21832-1-AP) by Proteintech is a Polyclonal antibody targeting ZP2 in WB, IF-P, ELISA applications with reactivity to human, mouse, rat samples 21832-1-AP targets ZP2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SKOV-3 cells, mouse brain tissue, mouse ovary tissue, rat brain tissue Positive IF-P detected in: mouse ovary tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information ZP2 is a major component of the zona pellucida to which sperm bind prior to penetrating the zona matrix and fusing with the egg. Following fertilization and cortical granule exocytosis, ZP2 is cleaved by ovastacin after which sperm do not bind to the zona pellucida. The zona pellucida and ovastacin proteins transverse the endomembrane system during oogenesis during which passage ZP2 remains intact. Secreted ZP2 is incorporated into the extracellular zona pellucida surrounding ovulated eggs and is cleaved in the zona matrix surrounding 2-cell embryos. (PMID: 28114310) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16386 Product name: Recombinant human ZP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 440-745 aa of BC096304 Sequence: EIHALWTDFPPSKISRDSEFRMTVKCSYSRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQPYGENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQWNVVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSSLVYFHCSALICNRLSPDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGPILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGAMDTKGHKTAGDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH Predict reactive species Full Name: zona pellucida glycoprotein 2 (sperm receptor) Calculated Molecular Weight: 745 aa, 82 kDa Observed Molecular Weight: 75-82 kDa GenBank Accession Number: BC096304 Gene Symbol: ZP2 Gene ID (NCBI): 7783 RRID: AB_3669375 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q05996 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.