Proteintech, 21877-1-AP, RSK4 Polyclonal antibody

Size: 20ul / 150ul The RSK4 (21877-1-AP) by Proteintech is a Polyclonal antibody targeting RSK4 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 21877-1-AP targets RSK4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IP detected in: MCF-7 cells, PC-12 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Ribosomal s6 kinase4 (RSK4), an X-linked gene, firstly was found as to be a potential tumor suppressive gene in a variety of cancers and is widely participated in signaling pathway. RSK4 is a predominantly cytosolic protein with very low expression and several characteristics of the RSK family kinases, including the presence of two functional kinase domains and a C-terminal docking site for ERK (PMID: 39114716, 30579335). Endogenous RSK4 showed the same level of phosphorylation of Ser232, Ser372, and Ser389 in starved and growth factor-stimulated cells, and mutational analysis demonstrated that these sites are important in stimulation of kinase activity (PMID: 15632195). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16299 Product name: Recombinant human RPS6KA6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 629-745 aa of BC148802 Sequence: TPEEILLRIGNGKFSLSGGNWDNISDGAKDLLSHMLHMDPHQRYTAEQILKHSWITHRDQLPNDQPKRNDVSHVVKGAMVATYSALTHKTFQPVLEPVAASSLAQRRSMKKRTSTGL Predict reactive species Full Name: ribosomal protein S6 kinase, 90kDa, polypeptide 6 Calculated Molecular Weight: 745 aa, 84 kDa Observed Molecular Weight: 84 kDa GenBank Accession Number: BC148802 Gene Symbol: RPS6KA6 Gene ID (NCBI): 27330 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UK32 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.